Taq DNA Polymerases
Polymerases are enzymes that occur in all living organisms and catalyze the polymerization of nucleotides. Their function is the replication, i.e. the multiplication of the DNA in the S-phase, which is a precondition for the transmission of genetic material in the cell division.
PANScript DNA Polymerase
PANScript is widely used by molecular biologists that have come to depend upon the robust performance of this reagent. PANScript is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates, and is the ideal choice for most assays.
PANScript is a robust preparation and consistently delivers high yields with minimal background. PANScript possesses 5‘ to 3‘ exonuclease activity and leaves an “A” overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
PANScript red DNA Polymerase
PANScript red DNA Polymerase is a formulation of our regular PANScript DNA Polymerase, which contains a non-toxic and non-hazardous red dye. The red dye provides an easy and quick identification of reactions to which the enzyme has been added, and facilitates the confirmation of complete mixing.
When the reaction is complete, a sample of the reaction mix can be loaded directly onto the agarose gel without the need for loading buffer, since the mix is of sufficiently high density to sink to the bottom of the gel.
The red dye migrates towards the positive electrode, thereby providing a means to monitor the progress of the electrophoresis. The presence of the dye has no effect on routine enzymatic manipulations, although rare exceptions may occur. In order to produce a reaction of sufficient density to allow for the direct loading of a sample onto a gel, we recommend using a minimum of 1.5 Units per 50 μl reaction.
PAN Hot Start DNA Polymerase
PAN Hot Start is a heat-activated thermostable DNA polymerase isolated from a novel organism. PAN Hot Start provides improved specificity as compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products.
PowerScript DNA Polymerase short range
Powerscript short DNA Polymerase is a high-performance proprietary complex of enzymes specifically designed for difficult/problematic PCR applications requiring high processivity with fidelity that would normally fail with standard Taq Polymerases. PowerScript short DNA Polymerase is recommended for short genomic DNA fragments of up to 3 Kb, or up to 5 Kb on Lambda DNA.