Reagents
Product details
Reagents
Our products are produced with the highest standards made in Germany. We guarantee constant quality and full traceability. Furthermore, we also offer professional solutions for everthing to do with cell culture.
Introduction
Introduction
PAN-Seratech manufactures and supplies a wide range of high quality reagents for use in cell culture. These reagents are supplied as a seperate product group for use in conjunction with PAN-Seratech serum products, cell culture media and buffer solutions.
Packaging
All liquid reagents are sterile filtered into gamma irradiated PET bottles. All reagents supplied in powder form are filled into convenient sized containers.
Storage
Exact storage conditions are shown on each product label and in the product specification as shown in this catalogue. Please ensure you take careful note of the storage conditions to ensure product integrity. All products are subject to a final quality control check before being packaged and despatched.
Media Supplements
Media Supplements
Maximum efficiency without loss of quality
Maximum efficiency without loss of quality PAN-Seratech reagents are tested according to the highest possible quality standards. All liquid reagents are dissolved according to in-house specifications, sterilized and filtered at 0.2 μm. Before final release, the reagents undergo extensive quality tests e. g. sterility, pH value, osmo.
Different Media Supplements
| Description | Size | Product number |
|---|---|---|
| HAT supplement (50x) | 100 ml | ST07-02100 |
| HT supplement (50x) | 100 ml | ST07-01100 |
| HEPES buffer 1M | 100 ml 500 ml | ST05-01100 ST05-01500 |
| HEPES - Sodium salt | 100 g 500 g | ST05-01100P ST05-01500P |
| Sodium bicarbonate 7.5 % | 100 ml | ST04-44100 |
| Sodium pyruvate 100 mM | 10 ml | ST04-43100 |
| ITS Solution I (100x) | 5 ml 10 ml | ST07-03100 ST07-03110 |
| ITS Solution II (100x) | 5 ml 10 ml | ST07-03200 ST07-03210 |
| ITS solution IV (100x) (w: Linoleic acid, BSA) | 5 ml 10 ml | ST07-03400 ST07-03410 |
| Insulin human rec. 10 mg/ml solution | 10 ml | ST07-04300 |
| Insulin human recombinant | 100 ml | ST07-04200 |
| Sterile Water for cell culture | 500 ml 1 L 20 L | ST04-991500 ST04-991000 ST04-992000 |
| β-Mercapthoethanol 50 mM in PBS | 20 ml 100 ml | ST07-05020 ST07-05100 |
| Pluronic F-68 10 % | 100 ml | ST08-02100 |
| Human Transferrin apo | 100 mg 500 mg 1 g | ST06-21100 ST06-21500 ST06-21000 |
| Demecolcine Solution 10 μg/ml | 10 ml | ST07-91010 |
| Sodium Chloride Solution 0.9 % | 500 ml | ST05-39500 |
Buffered Salt Solutions
Buffered Salt Solutions
PAN-Seratech offers a broad range of high quality buffered salt solutions. You can find more information on the appropriate sites.
DPBS Dulbecco`s Phosphate Buffered Salt Solution
| EBSS Earl`s Buffered Salt Solution
| HBSS Hank`s Balanced Salt Solution
|
PSS Puck`s Salt Solution A
| GBSS Gey`s Balanced Salt Solution
| TSS Tyrode`s Salt Solution
|
Amino Acids and Vitamins
Amino Acids and Vitamins
| Description | Size | Product number |
|---|---|---|
| BME Amino Acids Solution (50x), - without L-Glutamine | 100 ml | ST08-2000 |
| L-Glutamine 200 mM | 50 ml 100 ml | ST04-80050 ST04-80100 |
| Stable L-Glutamine 200 mM - (L-Alanyl-L-Glutamine) | 50 ml 100 ml | ST04-82050 ST04-82100 |
| L-Glutamine - Powder | 25 g 100 g 500 g | ST04-80025P ST04-80100P ST04-80500P |
| Stable Glutamine Powder - (L-Alanyl-L-Glutamine) | 10 g | ST04-82010P |
| MEM Amino Acids Solution (50x), - without L-Glutamine | 100 ml | ST08-30100 |
| MEM Amino Acids Solution (50x), - with L-Glutamine | 100 ml | ST08-31100 |
| MEM NEAA (100x) | 100 ml | ST08-32100 |
- Vitamins
| Description | Size | Product number |
|---|---|---|
| BME vitamin solution (100x) | 100 ml | ST08-40100 |
| MEM (100x) vitamin solution (100x) | 100 ml | ST08-41100 |
Antibiotics and Antifungal Drugs
Antibiotics and Antifungal Drugs
| Description | Size | Product number |
|---|---|---|
| Amphotericin B - 250 μg/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-01001 ST06-01005 ST06-01050 ST06-01100 |
| Amphotericin B - Powder | 50 mg 100 mg | ST06-01050P ST06-01100P |
| Amphotericin B water soluble - Powder | 25 mg 50 mg | ST06-01225P ST06-01250P |
| Bacitracin - Powder | 10 g 25 g | ST06-02010P ST06-02025P |
| Gentamicin sulfate - 10 mg/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-03001 ST06-03005 ST06-03050 ST06-03100 |
| Gentamicin sulfate - 50 mg/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-13001 ST06-13005 ST06-13050 ST06-13100 |
| Gentamicin sulfate - Powder | 1 g 10 g 25 g | ST06-03001P ST06-03010P ST06-03025P |
| Hygromycin B - 50 mg/ml | 20 ml 100 ml | ST06-08020 ST06-08100 |
| Hygromycin B - Powder | 50 mg 1 g | ST06-080050P ST06-080100P |
| Kanamycin sulfate - 5 mg/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-04001 ST06-04005 ST06-04050 ST06-04100 |
| Kanamycin sulfate - 10 mg/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-14001 ST06-14005 ST06-14050 ST06-14100 |
| Kanamycin sulfate - 50 mg/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-15001 ST06-15005 ST06-15050 ST06-15100 |
| Kanamycin sulfate - Powder | 10 g 50 g | ST06-04010P ST06-04050P |
| Minocyclin 0.5 mg/ml | 50 ml 100 ml | ST06-05050 ST06-05100 |
| Mycorase | 100 ml | ST06-02100 |
| Neomycin sulfate - 10 mg/ml | 50 ml 100 ml | ST06-06050 ST06-06100 |
| Neomycin sulfate - Powder | 10 g 25 g 100 g | ST06-06010P ST06-06025P ST06-06100P |
| Nystatin solution - 10,000 Units/ml | 100 ml | ST06-07800 |
| Paneticin 420 50 mg/ml | 20 ml 100 ml | ST06-16020 ST06-16100 |
| Paneticin G418 50 mg/ml | 20 ml | ST06-16220 |
| Paneticin 420 - Powder | 1 g 5 g 10 g | ST06-16001P ST06-16005P ST06-16010P |
| Penicillin/Streptomycin - 10,000 Units Penicillin/ml - 10 mg Streptomycin/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-07001 ST06-07005 ST06-07050 ST06-07100 |
| Penicillin/Streptomycin/Amphotericin B Mix - 10,000 Units Penicillin/ml - 10 mg Streptomycin/ml - 25 μg Amphotericin B/ml in 0.85 % saline | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-07301 ST06-07305 ST06-07350 ST06-07300 |
| Penicillin G potassium salt - Powder | 25 g 100 g | ST06-08025P ST06-08100P |
| Streptomycin sulfate - Powder | 25 g 50 g 100 g | ST06-11025P ST06-11050P ST06-11100P |
| Polymyxin B sulfate 10,000 Units/ml | 50 ml | ST06-09050 |
| Tiamulin 1 mg/ml | 50 x 1 ml 50 x 5 ml 50 ml 100 ml | ST06-12001 ST06-12005 ST06-12050 ST06-12100 |
| Zeocin 100 mg/ml | 10 ml | ST06-28010 |
Mycorase
Mycorase
Mycorase has been developed to remove a broad range of different strains of mycoplasma in most cell types. Mycorase is easy to use and does not affect eukaryotic cell proliferation. It is a highly effective antibiotic solution for safe eradication of mycoplasma contamination.
Special advantages
Ready-to-use solution
Effective removal of mycoplasma
No effect on cell proliferation
Broad range of action
Permanent cure for most cell types
| Description | Size | Product number |
|---|---|---|
| Mycorase | 100 ml | ST06-02100 |
Enzymes for Cell Dissociation
Enzymes for Cell Dissociation
PAN-Seratech offers a broad range of high quality enzymes for cell dissociation. You can find more information on the appropriate sites.
Collagenase
| Accutase®
| Trypsin and others
|
Attachment Factors
Attachment Factors
- PAN-Seratech offers a broad range of high quality attachment factors. You can find more information on the appropriate sites.
Collagen A
0.1 % Collagen A solution, 1 mg/ ml in HCl, type 1 For coating culture flasks with monometric Collagen A | Collagen R
0.2 % sterile solution Type 1 rat tail collagen; 2 mg/ml in 0.1 % acetic acid. Excellent substrate for the culture of hepatocytes, fibroblasts and epithelial cells.
0.4 % sterile solution Type 1 rat tail collagen; 4 mg/ml in 0.1 % acetic acid. Excellent substrate for the culture of hepatocytes, fibroblasts and epithelial cells.
| Gelatine Solution
|
| More information | More information |
|
Laminin Mouse
This highly purified preparation of mouse Laminin I increases cell adhesion, migration, growth, and differentiation. It is composed of 111 chains with a total MW of 800 kD and is used for the coating of culture dishes. | Fibronectin
Fibronectin is a large glycoprotein widely distributed in soluble form in the plasma and body fluids. Many cell types synthesize fibronectin. There is also an insoluble form of fibronectin in tissues. Plasma fibronectin is not identical to cellular fibronectin but equally effective in supporting cell attachment. Fibronectin promotes the attachment and spreading of many adherent cells on plastic, but also mediates binding to other extracellular matrix components such as e.g. collagen. | |
| More information | More information |
|
Transfection reagents
Transfection reagents
PAN-Seratech offers multi-purpose transfection reagents. You can find more information on the appropriate sites.
| Description | Size | Product number | |
|---|---|---|---|
PANFect A - especially low cytotoxicity | 1.0 ml | ST02-8010 | More information |
PANFect A-plus - enhanced transfection efficiency using less reagent | 1.0 ml | ST02-8110 | More information |
PANFect siRNA - for best performance in siRNA | 1.0 ml | ST02-8210 | More information |
PANFect kit - find the suitable transfection reagent with the kit | 10 x 0.1 ml | ST02-8000K |
All our PANFect reagents work only with the original PANFect Buffer!
This is supplied with each transfection reagent.
| Description | Size | Product number |
|---|---|---|
| PANFect Buffer Solution for 0.2 ml Transfection Reagent | 20 ml | ST02-8020 |
| PANFect Buffer Solution for 1.0 ml Transfection Reagent | 50 ml | ST02-8050 |
Separating Solutions
Separating Solutions
Pancoll
In many cases the isolation of cells is the first step for gene expression studies or in diagnostic procedures. Besides biological separation techniques physical separation methods are most commonly used. These methods use physical differences such as size and weight of the particles to be separated. For this purpose so-called separating solutions (= centrifugation media) are used.
Our Pancoll separating solutions contain a polysaccharide with a molecular weight of 400,000 daltons; this hydrophilic polymer allows for production of aqueous solutions for cell separation with a density of up to 1.2 g/ml. PAN-Seratech offers a variety of ready-to-use products with a density of 1.063 g/ml up to 1.091 g/ml for a very wide range of cell separation applications.
| Description | Size | Product number |
|---|---|---|
| Pancoll human, density 1.077 g/ml | 100 ml 500 ml | ST04-60100 ST04-60500 |
| Pancoll human for granulocytes, density 1.119 g/ml | 100 ml 500 ml | ST04-60110 ST04-60150 |
| Pancoll mouse, density 1.086 g/ml | 100 ml 500 ml | ST04-64100 ST04-64500 |
| Pancoll rat, density 1.091 g/ml | 100 ml 500 ml | ST04-65100 ST04-65500 |
| Pancoll animal, density 1.077 g/ml | 100 ml 500 ml | ST04-63100 ST04-63500 |
| Pancoll monocytes, density 1.068 g/ml | 100 ml 500 ml | ST04-68100 ST04-68500 |
| Pancoll platelets, density 1.063 g/ml | 100 ml 500 ml | ST04-67100 ST04-67500 |
- Storage
2° C to ambient temperature „Protect from light!“
When properly stored, separating solutions are stable for at least 36 months. The storage period starts with the manufacturing date.
Separating Solutions Pre-Filled
Separating Solutions Pre-Filled
Pancoll separating solutions from PAN-Seratech are made from a neutral, highly cross-linked, hydrophilic polymer of sucrose with an average molecular weight of 400,000 daltons. Pancoll is suited for separation of lymphocytes and other cell types. The ready-to-use solutions are available in 500 ml bottles (see page 105) as well as in pre-filled ready-to-use tubes with a separating membrane.
| Description | Size | Product number |
|---|---|---|
| Pancoll human, density 1.077 g/ml | 25 x 50 ml 50 x 10 ml | ST04-60125 ST04-60225 |
| Pancoll animal, density 1.077 g/ml | 50 x 10 ml | ST04-63225 |
- Stability
Pancoll is stable for 36 months at 2 °C to 20 °C. Protect from light!
- Method of seperation
For lymphocyte separation blood is used which has been defibrinated or treated with anticoagulants (Heparin, EDTA, Citrate), and which is diluted with the same volume of a physiological saline solution. Then the Pancoll solution is carefully covered with a layer of diluted blood in a centrifuge vial, without mixing the phases. After a short centrifugation step (e.g. 800-1000x g for 20-30 minutes without brake) at room temperature the lymphocytes, together with monocytes and platelets, can be harvested from the white blood cells layer between the plasma sample layer and the Pancoll.
The separated cells are then washed twice in physiological saline solution to purify the lymphocytes by removing platelets. During centrifugation the cells of the blood sample migrate to the Pancoll layer where they get into contact with the polysaccharide contained in Pancoll. The red blood cells are aggregated by this substance at room temperature immediately. Aggregation causes an increase of the sedimentation rate of the red blood cells which aggregate together with the granulocytes as a sediment at the bottom of the centrifuge vial.
Lymphocytes, monocytes and platelets are not so dense and can not enter and pass through the Pancoll layer. These cells are concentrated as white blood cell layer above the Pancoll layer and therefore can be harvested easily by careful pipetting. In subsequent centrifugation steps the lymphocytes are washed to remove remaining platelets, serum and Pancoll. As a result of this process a highly purified suspension of viable lymphocytes and monocytes (PBMC) is obtained.
PAN SL-S Product Line
PAN SL-S Product Line
Endothelial cell biology has been greatly advanced by studying cultured vascular endothelial cells in vitro. Besides the understanding of many physiologic and pathologic processes, a multitude of basic cell signalling processes has been elucidated by using endothelial cells in culture. Traditionally, complete endothelial growth media contain animal serum. The advance of so-called low-serum media for endothelial cells has improved the quality of experimental data acquired in recent years. However, endothelial cells may synthesize substances which can not be detected due to their low quantity or masking effects from serum.
In the past, cellular signalling pathways in endothelial cells have not been decipherable experimentally because even low concentrations of serum present in traditional media induce an undefined and undesired stimulation of cell surface receptors or intracellular signalling which only may become evident under serum-free conditions. As endothelial cells move into the field of interest for vascular tissue engineering with potential therapeutic application, the presence of whole animal serum is undesirable for such applications in the future.
All products described below are intended for use in a serum-free Endothelial Cell Culture System. Endothelial cells from different sources may be employed. For convenient use in this system, PAN-Seratech offers endothelial cells from human umbilical cord strictly isolated und cultured under animal serum-free conditions. This exclusive cell culture system is optimized for the maintenance and expansion of endothelial cells under serum-free conditions. Information about the composition, suitability, special advantages, and instructions are given for each individual product.
Panexin SL-S is a genuine serum substitute which can fully replace FBS in otherwise completely supplemented endothelial cell culture media. SL-S Trypsin/EDTA has been specifically designed for use in serum-free cell culture systems. A relatively low activity of trypsin results in gentle detachment of endothelial cells;
a low phenol red concentration acts as a convenient indicator while providing mild conditions for the cells. SL-S Medium is a working medium for rinsing of culture vessels after trypsin reaction to ensure a complete harvest of cells or for short time incubation of endothelial cells in growth factor free conditions. This medium is not suited for growth or long term culture of endothelial cells. SL-S Trypsin Inhibitor is optimized to stop trypsin reaction and simultaneously providing ideal conditions for endothelial cells to recover from trypsin activity. SL-S Collagen has been developed as a ready-touse solution for the coating of new culture vessels in endothelial subculture. SL-S Cryopan is a serum-free medium for cryoconservation of endothelial cells resulting in high rates of recovery of viable cells after thawing.
| Description | Size | Product number |
|---|---|---|
| Panexin SL-S Serum Substitute for HUVEC cultures | 25 ml | ST04-90065S |
| SL-S Trypsin/EDTA | 50 ml | ST10-0231SF |
| SL-S Trypsin-Inhibitor | 50 ml | ST10-0331SF |
| SL-S Medium (Working Medium) | 500 ml | ST04-300500 |
| SL-S Collagen 0.01 % | 25 ml | ST06-20650 |
| SL-S Cryopan | 25 ml | ST07-94050 |
Cryo Preservation
Cryo Preservation
PAN-Seratech offers a broad range of high quality cryo preservation. You can find more information on the appropriate sites.
DMSO
| Freezing Medium
Our freezing medium is recommended for the cryoconservation of cells. The medium is based on DMEM, supplemented with a mix of fetal bovine serum and DMSO. This composition guarantees a high survival rate and excellent cell growth after thawing.
| Crypopan I
Cryopan I is a serum-free freezing medium for the cryoconservation of cells in a nitrogen storage tank or an ultra-low temperature freezer (< -150° C). It contains DMSO.
|