Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. The production of bovine serum at PAN-Seratech is tightly controlled, from the collection of serum at the slaughterhouse and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany.

Fetal Bovine Sera

Fetal Bovine Serum from different countries of origins is sterile filtered and quality tested.

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• FBS Supreme

• Description	Size	Product number
  FBS Supreme, South America origin, fetal bovine serum, 0.2 µm sterile filtered	500 ml 100 ml	ST30-3031 ST30-3030

   

• Pansera ES

Our specially developed, proprietary processing methodology for serum enables us to offer a special fetal bovine serum for embryonic stem cells (ES).

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• Premium plus

Bovine Sera with the highest quality standard. Reduced precipitation.

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• Premium Grade

FBS made from the best fetal bovine serum. Suitable for a broad range of cell lines.

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• Standard Grade

FBS with the great cost efficiency. Suitable for a broad range of standard cell lines for research.

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• Bovine Sera

Bovine Serum from different countries of origins is sterile filtered and quality tested.

PAN-Seratech offers special types of serum: charcoal absorbed, delipidized, dialyzed, gamma irradiated, heat inactivated and gamma globulin reduced.

* every origin is possible

Activated charcoal serum

Fetal bovine serum is heated in a water bath with dextran and activated charcoal. The activated charcoal, together with the substances bound to it, is then removed by centrifugation and filtration. Application   Work involving reduced hormone content (steroids)   Work involving reduced growth factors (prevention of cell differentiation)   Receptor studies (e. g. estrogens)   Minimizes lot-to-lot variations in serum

Dialyzed serum
Serum is dialyzed with a 10,000 Dalton exclusion membrane against physiological saline solution (alternative DPBS) until the glucose content is below 10 mg/100 ml. Application   Radioactive labeling studies   Hormone-free applications   Tests intolerant for small molecules such as nucleotides (hypoxanthine, thymidine), amino acids (serine, alanine etc.), sugars or metabolites Delipidized serum

Delipidzed serum
Lipids are removed from serum by affinity chromatography. Application   Lipid metabolism studies   Arteriosclerosis research

Gamma irradiated serum
Serum is exposed to irradiation 25 kGy Application   Biopharmaceutical production   Virus production   Vaccine production   Manufacturing of diagnostic products

Heat inactivated serum

Serum is heated for 30 min to 56 °C in a water bath under repeated gentle mixing. Application   Measurements of lactate dehydrogenase in the culture supernatant as a marker for cell damage (serum LDH is inactivated by heat)   Minimizes lot-to-lot variations in serum (all thermolabile components are removed)   Studies on vitamins and growth factors   Enhance viral safety, since heat-labile viruses are inactivated   Tests that do not tolerate presence of complement (complement destruction)

Ultra low igG serum
The average IgG level in fetal bovine serum is in the range ;of 70 to 330 μg/ml. The IgG content in our Ultra low IgG serum is reduced by affinity chromatography (protein-G affinity column) to max. 5 μg/ml. The biological activity of serum is not affected. Application   Antibody production   Monoclonal antibodies   Radioactive labeling

Tetracycline-free serum
Fetal bovine serum is tested for absence of tetracycline using the TET-off system (luciferase). Application   TET-on / TET-off regulated gene expression   Transfections   Expression studies

The FBS Good product family contains specially processed serum products. Serum of selected batches is filtrated and separated into individual components by a sophisticated chromatographic method. The growth promoting components contained in the serum are then combined and restored in a defined process. Compared to conventional fetal bovine serum the FBS Good product family has been shown to support and promote cell growth of many different cell types equally well or even better.

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• Advantages

  Innovative new products

  Minor batch to batch variation

  Once tested – always same quality

  No batch testing required

  No lot reservation required

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• FBS Good

By developing FBS Good we wanted to create a naturally defined serum with a sustained growth promoting property and a higher safety. FBS Good only contains serum of highest quality from defined countries as specified. It is not blended or enhanced by addition of growth factors or proteins.

FBS Good advantages

  Reproducible growth properties

  Very low endotoxin level

  Suitable for a great variety of cells

  Continuous quality control

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• FBS Good Forte

By developing FBS Good Forte we wanted to create a naturally defined serum with an increased growth promoting property and a higher safety. Therefore, additional growth fortifying compounds have been added to increase cell proliferation. FBS Good Forte only contains serum of highest quality from defined countries as specified. In addition, growth promoting and stabilizing compounds (e.g. proteins, salts, sugars, vitamins) have been added to further enhance the stability of the serum as well as the proliferation of many different cell types.

FBS Good Forte advantages

  Reproducible enhanced growth properties

  Very low endotoxin level

  Suitable for many different cell types

  Continued high quality

  No more batch testing required

Besides bovine serum PAN-Seratech offers also other animal serum as listed below.

PAN-Seratech is known as a renowned producer of sera for cell culture. Our customers benefit from our years of experience and highly specialized products.

From the collection to the final product

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• Human Sera is the future for human cell therapy.
  
  Therefore the highly sensitive material and is subject to stringent safety EU regulations. The sample collectionis taken from human, healthy donors in Europe, certified donations institutions. The average age of our donors is approximately 26 years. The collection and testing of human plasma is carried out in accordance with the current version of Good Manufacturing Practice Annex 14th.
  
  Each donation is precisely tested regarding various biophysical parameters and viral infections and negative in the tests anit HIV 1/HIV 2, Hbs Ag, anti-HCV, HIV-1 Ag and/or HIV-1 NAT and Syphilis. From the collection of the plasma up to the packaging of the final product each individual production step is controlled and documented. Therefore, we provide our customers characterized with sera of consistently high quality, an absolute maximum level of safety and transparency.
  
  In each batch of human serum among others the osmolarity, pH, protein- and glucose concentration, and cell growth are measured and the sterility tested.
  
  Human Sera produced by PAN-Seratech consist exclusively of human material. Human serum may be more suitable for the cultivation of human cells than FBS since serum and the cultured cells belong to the same species. Thus, the physiological and natural conditions in vitro are reproduced most similar to those in vivo. Human serum is particularly suitable for cell culture of human cells, tissue engineering of human tissue, human cell therapies (Clinical Trials I and II), particularly sensitive cell lines and immune cells.
  
  PAN-Seratech offers three different categories of human serum: "Off the clot", Seraclot and converted human serum. Within these three categories it is possible to choose between a mixed groups pool or human serum AB.
• Off the clot serum

Off-the-clot serum is prepared from human whole blood collected without anti-coagulant, allowed to clot at room temperature and then centrifuged to remove the clot. We provide single donor units as well as pooled off-the-clot serum. Off-the-clot serum is filtered through depth and membrane filters before filling.

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• Seraclot

• Seraclot is a human serum, which is equal to the „off-the-clot“ serum in cell culture and in many cases exceeds its quality. An increased content of growth factors expands the possibilities in therapeutic cell culture. Seraclot is excellently suited for tissue engineering and cell differentiation. Seraclot is composed exclusively of human material.
• Human Sera from PAN Seratech

Human serum is produced from human plasma by addition of calcium chloride. This results in clotting of the plasma. After the coagulation process the cellular and solid constituents of the serum are removed by centrifugation, the human serum is washed and concentrated by ultra-filtration and finally filtered through a combination of depth- and membrane- filters.

PAN-Seratech offers also converted human serum from donors with the blood group AB. Their blood does not contain anti-A nor anti-B antibodies, that could damage cultured cells and is therefore particularly suitable for immunological and hematopoietic studies.

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• Customer information

• Human serum is a natural product. After the sterile filtration it is bottled and frozen as a clear solution. After thawing a slight blur may occur. Thus does not mean any loss of quality and the serum can be added to the medium without concerns. Human serum is usually used in a concentration between 5% and 20%.
  
  Cells cultured with FBS should be adapted step by step to the new serum. Initially, the cells should be cultivated for 4-6 days with a 10% serum mixture, consisting of 5% FBS and 5% human serum. Afterwards the final conversion to human serum can take place.

PAN-Seratech offers a broad range of high qualitative albumins. You can find more information on the appropriate sites.

Other sizes and products are available upon request.

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• Bovine Serum Albumin

Albumins serve as additive proteins for tissue cultures. They are the main protein component in serum and are added to cell culture media to increase the stability of cell membranes and to bind possibly toxic elements.

Advantages

  High purity > 99 %

  Minimal lot to lot variation

  Stringent control of raw materials

  Minimal BSE/TSE risk

  US origin

  Full documentation

  Special products for unique applications

Storage

Powder and preserved solutions at 2 - 8 °C.
Preservative free solution at -20 °C

Other sizes and products are available upon request.

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• Human Serum Albumin

Human serum albumin is the most abundant protein in blood. Albumin transports hormones, fatty acids and other components. The main biological function is to regulate the colloidal osmotic pressure of blood. Human serum albumin e.g. can be used for protein calibration studies and solubilization of lipids.

Advantages

  Stringent control of raw materials

  Full documentation

Storage

Human Serum Albumin can be stored at 2 - 8 °C.